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Image Search Results
Journal: Drug Design, Development and Therapy
Article Title: MK-4 Ameliorates Diabetic Osteoporosis in Angiogenesis-Dependent Bone Formation by Promoting Mitophagy in Endothelial Cells
doi: 10.2147/DDDT.S503930
Figure Lengend Snippet: MK-4 ameliorates the impairments of type H vessel formation and angiogenesis-dependent bone formation in DOP mice. ( a ) Schematic drawing of the procedures conducted in this study. ( b and c ) Representative flow cytometry plots of CD31 hi Emcn hi ECs (type H ECs). The bar graph showed the quantitation of type H ECs. ( d and e ) Confocal image of CD31 hi and Emcn hi (type H blood vessels) in mice models and histomorphometric quantitation of the area of type H blood vessels. Scale bar: 50μm. ( f and g ) Confocal image of Osterix + (green) osteoprogenitors around CD31 hi vessels (red) and the quantitative analysis. Scale bar: 50μm. ( h ) Typical three-dimensional and two-dimensional coronal images of the tibias were obtained by Micro-CT. ( i ) HE and Masson staining of mice tibias. Scale bar: 200μm or 100μm. ( j ) Statistical analysis of bone histological parameters. ( k and l ) IHC analysis of ALP and RUNX2 in the tibias of mice models. Scale bar: 50μm. Data were presented as mean ± SD (n=5). * P < 0.05. ** P < 0.01. *** P < 0.001.
Article Snippet: The cells were subsequently washed and later incubated with PE-conjugated CD45 (E-AB-F1136D, 1:20; Elabscience, Wuhan, China) and
Techniques: Flow Cytometry, Quantitation Assay, Micro-CT, Staining
Journal: Drug Design, Development and Therapy
Article Title: MK-4 Ameliorates Diabetic Osteoporosis in Angiogenesis-Dependent Bone Formation by Promoting Mitophagy in Endothelial Cells
doi: 10.2147/DDDT.S503930
Figure Lengend Snippet: 3-MA partially counteracts the promotion of type H vessel recovery and angiogenesis-dependent bone formation gain by MK-4. ( a and b ) Confocal image of CD31 hi and Emcn hi (termed type H blood vessels) in mice models and histomorphometric quantitation of the area of type H blood vessels. Scale bar: 50μm. ( c and d ) Confocal image of Osterix + (red) osteoprogenitors around Emcn hi vessels (green) and the quantitative analysis. Scale bar: 50μm. ( e ) HE and Masson staining of mice tibias. Scale bar: 200μm or 100μm. ( f ) Statistical analysis of bone histological parameters. ( g and h ) IHC analysis of ALP and RUNX2 in the tibias of mice models. Scale bar: 50μm. Data were presented as mean ± SD (n=5). * P < 0.05. ** P < 0.01. *** P < 0.001.
Article Snippet: The cells were subsequently washed and later incubated with PE-conjugated CD45 (E-AB-F1136D, 1:20; Elabscience, Wuhan, China) and
Techniques: Quantitation Assay, Staining